St Lukes Church is the Parish church for Charlton. It was built on the site of a previous church in 1630 with funds left by the owner of Charlton House, Sir Adam Newton. There is a monument to Sir Adam inside the church. The nave, south aisle, porch, and tower date from 1630, the north aisle from 1639. A new chancel was added in 1840.
Spencer Perceval, (1762-1812) the only British Prime Minister to be assassinated, is buried here, and there is a plaque and bust to him inside the church.